KMID : 0616120190500030198
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Korean Journal of Pharmacognosy 2019 Volume.50 No. 3 p.198 ~ p.204
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A Convenient Manufacturing Method for Mass Production of EGCG Rich Green Tea Extract
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Seo Eun-Hye
Kim Eun-Jeong Cheon Seong-Bong Yoon Min-Ji Choi Sang-Un Ryu Geon-Seek Ryu Shi-Yong
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Abstract
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A facile and convenient method was developed for the mass production of epigallocatechin gallate (EGCG) rich green tea extract (Er-GTE). The Er-GTE was successfully obtained from the crude water extract of green tea by the combination of two step purification, i.e., a simple adsorption process on the cation exchange resins (Trilite SCR-B) followed by the chromatography with Diaion HP-20 resins. The green tea extract produced by water extraction under 45oC was subjected to adsorb on the strongly acidic cation exchange resin, Trilite SCR-B. The eluate passed through the resin was reabsorbed on Diaion HP-20 resin, which was subjected to elute with a mixture of water and alcohol by conventional chromatographical manner. The EGCG content in Er-GTE was estimated above 97% by HPLC analysis and the newly developed method was regarded as the most suitable and appropriate process for the mass production of epigallocatechin gallate rich green tea extract (Er-GTE).
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KEYWORD
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Camellia sinensis, Epigallocatechin gallate, Green tea extract, Mass production
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